In vitro culture and investigation of human osteoblasts from alveolar bone
- VernacularTitle:人牙槽成骨细胞的体外培养和生物学特性检测
- Author:
Shaoyun JIANG
;
Rong SHU
- Publication Type:Journal Article
- Keywords:
Alveolar bone;
Osteoblasts;
Cell culture;
Human
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To culture human alveolar osteoblasts and study its osteogenic characteristics in vitro.Methods:Human alveolar bone was obtained from human donor and cultured in explants.Cells migrating from explants were observed by inverted phase-contrast microscope and cell proliferation was detected by MTT assay.After cultured in conditional medium containing dexamethasone,?-glycerphosphoric sodium and ascorbic acid,cells were tested by ALP assay,Alizarin red assay and von Kossa assay,3H-proline labeling with collagenase digestion method.Results:The alveolar osteoblasts migrated from explants after 5 d culture.Cells could be passaged after 14-16 d culture.ALP values were obviously positive and cells showed positive reaction by Alizarin red assay and von Kossa assay in conditional medium.The cultured human alveolar osteoblasts secreted type I Collagen.Conclusion:The human alveolar osteoblasts cultured in this experiment grow well,and the morphological and biological characteristics of the culture cells are similar to those of osteoblasts.
