The effects of LPS on the IL-6 expression in osteoblasts
- VernacularTitle:LPS对成骨细胞IL-6表达的影响
- Author:
Xiulan LI
;
Wei ZHAO
;
Chengzhi GAO
- Publication Type:Journal Article
- Keywords:
Osteoblasts;
RT-PCR;
Enzyme-linked immunosorbent assay;
IL-6
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of LPS on the IL-6 expression in osteoblasts.Methods:Rat calvarial osteoblasts were primarily cultured. LPS from Escherichia coli with different concentration were administrated. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to identify the IL-6 mRNA expression of the cells. The intensities of electrophoresis bands were measured. Enzyme-linked immunosorbent assay (ELISA) was used to detect IL-6 protein production. The data were analyzed with SPSS 10.0 software package.Results:E. coli LPS with concentrations of 1?10-5 g/L-1?10-3 g/L induced IL-6 mRNA and IL-6 protein expression in osteoblasts. Statistically significant differences were detected at concentrations 1?10-4,1?10-3 g/L E.coli LPS.Conclusion:LPS can increase the expression of IL-6 in osteoblasts in the gene and protein levels. IL-6 may play an important role in the alveolar bone metabolism.
