Effects of baicalin on proliferation and cell cycle of human periodontal ligament cells by stimulation of LPS in vitro
- VernacularTitle:中药黄芩苷对脂多糖介导人牙周膜细胞增殖的时间效应和细胞周期的影响
- Author:
Huiying LI
;
Xuecong LIU
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Baicalin;
Cell culture;
Periodontal ligament cells;
Flow cytometry;
MTT method
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Chinese herb baicalin on proliferation and cell cycle of periodontal ligament cells(PDLC) by stimulation of lipopolysaccharide(LPS) in vitro.Methods:Human PDLC was cultured in vitro.MTT colormetic method was used to evaluate the effects of Chinese herb baicalin on proliferation of PDLC by stimulation of LPS.Flow cytometry was used to analyze the possible DNA index and cellular cycle changes of PDLC.Results:PDLC showed a significant growth inhibition after being treaded with 100 ?g/ml LPS 3 h by MTT methods,and proliferation ability of PDLC improved greatly after adding baicalin.FCM results showed that the cell number increased in G1 phase,while the cell number decreased in S phase at 12 h after treated with 100 ?g/ml LPS,but the cell number decreased in G1 phase,and increased in S phase after treated with Chinese baicalin.Conclusion:Chinese herb baicalin can significantly promote the proliferation of PDLC.It can make some static cell in G1 phase enter S phrase and improve the growth of human PDLC.