In vitro culture and phenotype analysis of monocyte-derived dendritic cell from peripheral blood of patients with oral carcinoma
- VernacularTitle:口腔癌患者外周血树突状细胞的体外培养及表型分析
- Author:
Yuqin DUAN
;
Lijun CHENG
;
Lantao GUO
- Publication Type:Journal Article
- Keywords:
Mouth neoplasms;
Dendritic cell;
Cell factor;
Phenotype of cell
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the phenotype of induced dendritic cell (DC) derived from peripheral blood of the healthy individuals and the patients with oral carcinoma. Methods:15 patients with oral carcinomal at stages II or above were assigned to study group and 15 cases of healthy volunteers were assigned to control group. Leukocyte were isolated by density centrifugation. Adherent fractions were incubated in present of GM-CSF,IL-4 and TNF-? for 7 days in vitro. The morphological change of the cultured cells was observed by light microscopy and electronic microscopy. The phenotype expressions of CD83,CD1a,CD86, CD80, HLA-DR were analysized by flow cytometry. Results:Typical DCs were obtained from two groups after 7 days culture. The expression rate of CD83,CD1a on DC from healthy volunteers was significantly higher than that from patients (P0.05). Conclusion:DC derived from oral cancer patient' s peripheral blood monocyte might be more matured and is a good cell model for further immunotherapy and research.
