Effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat condylar chondrocytes
- VernacularTitle:体外周期性单轴压应力下髁突软骨细胞Raf激酶抑制蛋白(RKIP)的动态变化研究
- Author:
Huang LI
;
Song LI
;
Tuojiang WU
- Publication Type:Journal Article
- Keywords:
Compressive stress;
Mandibular condyle;
Chondrocytes;
Raf kinase inhibitor protein
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat condylar chondrocytes. Methods:The third-passage chondrocytes were harvested from the mandibular condyles of 2-day-old rats, and a cellular compressive stress device (self-made four-point bending system. Patent No: 01129166.4 & 01256849.x) was used to apply stress on cells at 4 000 ?strain ( 0.5 Hz frequency) for 0, 15, 30, 60, 120 and 240 min. The effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat mandibular condylar chondrocytes were examined by REAL-TIME PCR and Western blot. Results:The results showed that the expression of RKIP and its mRNA changed obviously with 4 000 ?strain compressive stress loading, there are reverse tendency in the expression between RKIP and its mRNA. RKIP mRNA significantly increased at 30min (P
