Expression of a lysozyme from antheraea pernyi in pastoris and the effects of recombinant Ap-lysozyme on Streptococcus mutans
- VernacularTitle:柞蚕溶菌酶基因在酵母中的表达及对变形链球菌的作用
- Author:
Dan WANG
;
Weidong MA
;
Huijuan YU
- Publication Type:Journal Article
- Keywords:
Recombinant AP-Lysozyme;
Streptococcus mutan;
Yeasts
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct the antheraea pernyi lysozyme(ApLZ) expression system using the methylotrophic yeast Pichia pastoris, to assay the antibacterial activity of the recombinant ApLZ against Streptococcus mutans. Methods:The ApLZ expression system was used in the expression and purity of Ap-lysozyme. The antibacterial activity of the recombinant ApLZ against Streptococcus mutans were assayed by using agar diffusion method. Results:Expression system of ApLZ was constructed using the methylotrophic yeast Pichia pastoris as a host. ApLZ was expressed correctly and secreted efficiently when the native signal sequence of ApLZ was used as secretion signal. The level of ApLZ expression in Pichia pastoris peaks at 96 h after the induction of sustaining 5 ml/L methanol. The molecular weight of the recombinant ApLZ is about 20 000. Conclusion:The recombinant ApLZ is active in inhibiting the growth of Streptococcus mutans.
