Analysis of gene expression profiles in Tca8113/CBP nude mice by microarray technique
- VernacularTitle:基因芯片对荷瘤裸鼠Tca8113/卡铂耐药基因表达谱的分析
- Author:
Ge FENG
;
Dazhang WANG
;
Huaiqing CHEN
;
Jia HE
;
Weidong LENG
- Publication Type:Journal Article
- Keywords:
Tca8113 cell;
Carboplatin(CBP);
Multidrug resistance(MDR);
Microarray
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the differences of gene expression between Tca8113 and Tca8113/CBP tissues in nude mice. Methods:Tca8113 cells were injected subcutaneously in both sides of armpits of nude mice at the concentration of 5?106 cells/0.1 ml. Two weeks after injection, Carboplatin was used subcutaneously around the tumor 0.01 mg/g (weight) each day in Tca8113/CBP group while Tca8113 group was injected with physiologic saline as control. Mice were sacrificed 10 weeks after drug injection. The two kinds of tissues were investigated by human 16k cDNA v2.1 SBC-R-HC-100-21 gene chip. Results:Among the 16 000 target genes, there were 719 genes whose expression levels showed differences between the two kinds of tumor tissues. Conclusion:Microarray technique can simultaneously screen different genes from above-mentioned two kinds of tissues. Further analysis of the obtained genes will be helpful to understand the molecular mechanism of multidrug resistance.
