Expression and antibody preparation of stretching sensitive gene transcription factor 4
- VernacularTitle:应力敏感基因转录因子-4的表达与抗体制备
- Author:
Xue FENG
;
Bin ZHANG
;
Yongming LI
;
Yin DING
;
Yinzhong DUAN
- Publication Type:Journal Article
- Keywords:
Mechanical stretch;
Transcription factor 4;
Antibody;
Cell
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the function of transcription factor 4 (TCF4) and to prepare TCF4 polyclonal antibody.Methods:pET-41/TCF4 was transformed into E.coli BL21(DE3) and induced by IPTG.The purified GST-TCF4 fusion protein was applied to immunize rabbit to produce antiserum. The specificity of the affinity of purified anti-TCF4 antibody was examined by Western blotting analysis of the eukaryotic expressed products of TCF4. Dig-labeled probe and antibody against TCF4 were used to examine the expression of TCF4 in Saos-2 cells under mechanical stretch. Results:Western blotting showed that the antibody could bind to TCF4 specifically. The expression of TCF4 mRNA and protein were significantly increased in Saos-2 cells under mechanical stretch. Conclusion: TCF4 antibody has been prepared successfully.
