Ultrastructure and lanthanum element in BMSCs after treatment by Lanthanum Chloride
- VernacularTitle:镧盐处理后骨髓基质干细胞内超微结构及镧元素分析
- Author:
Juan LIN
;
Xiaoping WANG
;
Haijiang LIU
- Publication Type:Journal Article
- Keywords:
Lanthanum;
Bone marrow stromal cells;
TEM;
Electron microprobe;
High resolution TEM
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether lanthanum(La 3+)can enter into ossification induced bone marrow stromal cells(BMSCs)cultured in vitro and its influences on the ultrastructure of BMSCs.Methods:Dog BMSCs were ossification induced and the cells of the third passage were cultuerd with the presence of lanthanum chloride(LaCl_3)at 5.564?102,5.564 and 5.564?10-2 ?g/ml respectively for 5 days,the same passage cells without LaCl_3 treatment were used as the controls.Transmission electron microscope(TEM),electron microprobe with wave spectrum(EMWS)and high resolution TEM with energy spectrum(HRTEM)were used to observe the change of BMSCs ultrastructure and the distribution of lanthanum ions in the cells.Results:Electron-dense granules in lysosome were observed in BMSCs treated with LaCl_3 but not in control BMSCs under TEM.Slight denaturation of some organelles was found in both LaCl_3 treated and control cells.EMWS examination showed that there was La element in BMSCs treated with 5.564?102 ?g/ml LaCl_3.La element was not found in all the treated cells under HRTEM.Conclusion:La 3+ at a certain concentration might enter into the ossification induced BMSCs cultured in vitro.
