The effect of 5-FU polylactic acid nanospheres on the proliferation and apoptosis of human tongue squamous cell carcinoma Tca83 cells
- VernacularTitle:5-FU聚乳酸纳米微球对人舌癌Tca83细胞增殖及凋亡的影响
- Author:
Chengyue WANG
;
Hongbo ZHAO
;
Xiguang LI
- Publication Type:Journal Article
- Keywords:
Polylactic acid nanospheres;
5-FU;
Tca83 cells;
Apoptosis
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of 5-FU polylactic acid nanospheres(5-FU-PAN)on the proliferation and apoptosis of the human tongue squamous cell carcinoma Tca83 cells.Methods:Tca83 cells were exposed to 5-FU-PAN or 5-FU at the doses(mmol/L)of 1?10-2,2?10-2 and 4?10-2 for 1,3,5,7,9 and 11 days respectively.The survival rates of the cells were examined by MTT assay,the apoptosis of the cells was observed by acridine orange fluorescent staining and the cell cycle distribution was studied by flow cytometry.Results:Both 5-FU-PAN and 5-FU inhibited the proliferation and promoted the apoptosis of the cells dose and time dependantly.5-FU-PAN showed stronger effects than 5-FU.The two agents blocked the cells at S phase and 5-FU-PAN was more potential than 5-FU.Conclusions:5-FU-PAN is more effective in the growth inhibition and apoptosis promotion of Tca83 cells.
