Expression of heat shock response protein induced by heat shock in human Tca8113 cells
- VernacularTitle:热休克诱导人舌鳞癌Tca8113细胞HSP_(70)表达的研究
- Author:
Lin XIAO
;
Xinhua LIANG
;
Jing HU
;
En LUO
;
Zuyi MAO
- Publication Type:Journal Article
- Keywords:
Tongue cancer Tca8113 cell;
Heat shock response;
Heat shock protein
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of heat shock response protein(HSP_ 70 ) in human squamous tongue cancer Tca8113 cells during the recovery periods of heat shock.Methods:Tca8113 cells were subjected to heat shock at 43 ℃ for 30 min, then the cells were cultured for 2,4,6,8,12,24 and 48 h respectively. The expression of HSP_ 70 in the cells was examined with immunohistochemical method, Quantitative analysis was performed by FCM and the cell vitality was detected by MTT method.Results:Heat shock induced HSP_ 70 expression in Tca8113 cells at 43 ℃ for 30 min and the maximum proportion of the positive cells were observed and HSP_ 70 reached the maximum value at 12 h after heat shock(P
