Effects of soluble CD14 on the activity of human gingival fibroblasts induced by lipopolysaccharide
- VernacularTitle:可溶性CD14对LPS诱导人牙龈成纤维细胞活化的影响
- Author:
Benxiang HOU
;
Jun YIN
;
Jia CAO
- Publication Type:Journal Article
- Keywords:
sCD14;
LPS;
Endotoxin neutralizing protein;
Human gingival fibroblasts
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of soluble CD14 (sCD14) on the inducing activity of lipopolysaccharide(LPS) on human gingival fibroblasts(HGFs). Methods:In vitro cultured HGFs were stimulated by FITC labeled LPS at the concentration of 0 (control), 100 ng/ml, 100 ng/ml LPS+100 ?g/ml sCD14 and 100 ng/ml LPS+100 ?g/ml ENP respectively for 24 h. The binding activity of LPS to HGFs was measured in the level of mean fluorescence intensity of FITC-LPS.TNF-? and IL-6 in the culture supernatant were measured by ELISA. Results:The binding activity of FITC-LPS to HGFs and the level of TNF-? and IL-6 in culture supernatant were significantly decreased in sCD14 and ENP group(P
