Effect of human p27~(kip1) gene transfection on the proliferation of human tongue squmaous cell carcinoma cells
- VernacularTitle:p27~(kip1)基因转染及其对人舌癌细胞增殖的影响
- Author:
Wenshu LIU
;
Ruhui ZHANG
;
Yajuan XU
;
Yongzhong XU
- Publication Type:Journal Article
- Keywords:
p27~(kip1);
Clone;
Squmaous cell carcinoma;
Transfection;
Cell cycle
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of human p27~(kip1) gene on the proliferation of human tongue squamous cell carcinoma(TSCC) Tca8113 cells.Methods:Human p27~(kip1) gene was cloned from normal tissue and reconstructed into eukaryotic expression vector pcDNA3, the recombinant plasmid was transfected into Tca8113 cells with liposome.The integration and expression of exogenous objective gene in the target cells were examined by PCR and flowcytometry,the growth of transfected cells and non-transfected cells was investigated by MTT assay and flowcytometry.Results:p27~(kip1) cDNA was successfully cloned and reconstructed into vector pcDNA3.The gene was trasfected into Tca8113 cells and stable transfection was achieved by screening with G418.After p27~ kip1 transfection the cell proliferation was inhibited,proliferation index decreased(P
