The effects of lipopolysaccharide on mCD14 expression in human gingival fibroblasts in vitro
- VernacularTitle:内毒素对牙龈成纤维细胞mCD14表达的影响
- Author:
Xianhua ZHANG
;
Jincai ZHANG
;
Yunhui ZHANG
;
Naimei YUAN
- Publication Type:Journal Article
- Keywords:
Human gingival fibroblasts;
Lipopolysaccharide;
mCD14
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the distribution of CD14 in human gingival fibroblasts (HGFs) and the effects of lipopolysaccharide(LPS) on mCD14 expression in HGFs in vitro. Methods:HGFs were in vitro cultured and exposed to LPS at 0.1-10 ?g/L.The expression of mCD14 in the cells was examined by immunohistochemistry staining and Western blot.Results:mCD14 was found on healthy human HGF cell membrane surface and LPS at 1 ?g/ml and 10 ?g/ml increased the expression of mCD14 in 1-12 h exposure.Conclusion:LPS may regulate mCD14 expression of HGFs.
