The effect of hypoxia on uPA activity in human tongue carcinoma cell line Tca8113
- VernacularTitle:乏氧对人舌癌Tca8113细胞uPA活性的影响
- Author:
Xinhua LIANG
;
Yongwen HE
;
Lin XIAO
;
Zuyi MAO
;
Xian LIU
;
Chunxu ZHANG
- Publication Type:Journal Article
- Keywords:
Hypoxia;
Urokinase-type plasminogen activator(uPA);
Tongue;
Squamous cell carcinoma
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the activity of urokinase-type plasminogen activator (uPA) in human tongue squamous cell carcinoma cell line Tca8113 under hypoxia in vitro. Methods:According to the different time of hypoxia, the test was divided into four groups as following: (1) 0 h; (2) 6 h;(3)12 h;(4)24 h. The expression of uPA was examined using immunochemistry. The expression of uPA mRNA was examined using in situ hybridization (ISH). The results of ISH staining of uPA mRNA were analysed using Spot and IPP image analysis system.Results:The uPA expression was found in cytoplasm and cytomembrane of Tca8113 cells.uPA mRNA expression was found in cytoplasm of the cells.The staining intensity of uPA mRNA in the groups of (1),(2),(3) and (4) was 0.175 251?(0.013) 863,0.263 191?(0.000 680),0.406 745?0.014 016 and 0.478 919?0.018 998 respectively(P
