Cytotoxicity of dental bonding agents to human dental pulp cells in vitro
- VernacularTitle:牙本质粘结剂对人牙髓细胞毒性的体外研究
- Author:
Guo ZHANG
;
Cui HUANG
;
Zhixing ZHANG
;
Tieli ZHENG
;
Sa WANG
;
Xiangrong CHENG
- Publication Type:Journal Article
- Keywords:
Dental adhesive;
Dental acid etching;
Cytotoxicity;
Pulp cell
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the cytotoxicity of different dental bonding agents to human dental pulp cells. Methods:Four dental bonding agents(Prime & Bond NT,Single Bond,Xeno Ⅲ and iBond) were diluted with the culture medium by a ratio of 1∶1 000,1∶2 000 and 1∶4 000(v/v), and the fourth passage of dental pulp cells were then exposed to agent dilutions for 12, 24 and 48 h respectively, then the morphological changes of pulp cells were observed with microscopy and cytotoxicity was analyzed with modified 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay.Results:There was significant influence of the type of dental bonding agents,exposure time and concentration on the cell morphology. Total-etch system was more toxic than self-etch system.Conclusion:Dental bonding agents are toxic to pulp cells,Single Bond is the strongest cytotoxic agent among the 4 adhesive agents.
