The effects of enamel matrix proteins on biological characteristics of human dental follicle cells in vitro
- VernacularTitle:釉基质蛋白影响人牙囊细胞生物学特性的实验研究
- Author:
Zhen WANG
;
Hongwei LIU
;
Yan JIN
;
Lanning LIU
- Publication Type:Journal Article
- Keywords:
Dental follicle cells;
Enamel matrix proteins;
Proliferation;
Alkaline phosphotase
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of enamel matrix proteins(EMPs) on proliferation and alkaline phosphotase (ALP) activity of human dental follicle cells(HDFCs) in vitro. Methods:HDFCs were dissected and cultured by digestion with bacterial collagenase. MTT method and enzyme kinetics method were used to examine the proliferation and ALP activity of HDFCs treated with EMPs.Results:EMPs at 25~200 mg/L increased the proliferation of HDFCs and EMPs at 100 mg/L showed the strongest effects in 7-day-culture.EMPs at 100 mg/L increased ALP activity of HDFCs in 3- and 7-day cultures.Conclusion:EMPs may stimulate the proliferation and the ALP activity of HDFCs.
