Effect of Slit-Robo signal on the proliferation of oral cancer cell line Tb
- VernacularTitle:Slit-Robo信号对口腔癌Tb细胞增殖作用的影响
- Author:
Yuguang MA
;
Lijing WANG
;
Bing HAN
- Publication Type:Journal Article
- Keywords:
Slit-Robo signal;
Tb cell;
R5 mAb;
Proliferation
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of Slit-Robo signal on the proliferation of human oral squamous cell carcinoma line Tb. Methods:The expression of Slit and Robo protein was measured with Western blot. After the addition of the monoclonal antibodies (mAb) of R5 against Robo1 receptor extracellular domain, the changes of cell proliferation were studied by MTT assay and clone formation assay,the expression of PCNA was measured with immunohistochemical staining. Results:Western blot showed that Slit and Robo proteins were expressed in Tb cells. The proliferation rate of Tb cells decreased and the expression of PCNA decreased after the addition of R5 mAb. Conclusion:Slit-Robo signal may regulate the proliferation of Tb cells by up-regulation of PCNA.
