Appraisement of multidrug resistance of a human salivary adenoid cystic carcinoma cell line
- VernacularTitle:顺铂诱导人涎腺腺样囊性癌细胞多药耐药性的评价
- Author:
Bo YANG
;
Yinhui TAN
;
Jianshe ZHANG
- Publication Type:Journal Article
- Keywords:
Salivary;
Adenoid cystic carcinoma;
Multidrug resistance
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a multidrug resistant human salivary adenoid cystic carcinoma cell line. Methods:Salivary adenoid cystic cells of the cell line SACC were exposed to 1 ?g/ml of DDP for 48 h at the interval of one month. 6 months later, the cell line was established and named SACC/DDP. MTT method was used to study the drug resistance of SACC and SACC/DDP cells against 4 chemotherapeutic agents(MTX, PYM, VCR, MMC). Flow cytometry was adopted to study the cell cycle distribution of the cells after treatment with 4 agents at different concentrations for 72 h. Results:The drug resistance of SACC/DDP cells against VCR,MTX,PYM and MMC was 2.9,2.4,1.1 and 1.8 times of that of SACC cells. The ratio of G0 cell number to G1 cell number in SACC/DDP cell line was smaller than that in SACC cell line after treatment with the 4 agents at different concentrations respectively.Conclusion:The multidrug resistant human salivary adenoid cystic carcinoma cell line can be estabished by exposing parent cell line SACC periodically to DDP.
