The effects of 17 beta-estradiol on the proliferation and metastasis of salivary mucoepidermoid carcinoma M_3SP_4 cells
- VernacularTitle:17?-雌二醇对涎腺黏液表皮样癌高转移细胞M_3SP_4的作用
- Author:
Fuping GUO
;
Junzheng WU
;
Jing WANG
- Publication Type:Journal Article
- Keywords:
Salivary gland;
Mucoepidermoid carcinoma;
17beta-estradiol
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of 17 beta-estradiol (E2) on the proliferation and metastasis potential of salivary gland mucoepidermoid carcinoma M_3SP_4 cells. Methods:The effects of E2 on the potential of proliferation and metastasis of M_3SP_4 cells were investigated by cell counting, MTT assay, clonegenetic assay, in vivo tumour growth and metastasis assay in nude mice.The expression of VEGF,c-erbB-2,Ki-67 and P16 in M_3SP_4 cells was examined by immunohistochemistry assay. Results:E2 increased the proliferation of M_3SP_4 cells at 10~-10-10~-5 mol/L. E2 at 10~-7 mol/L showed the strongest effects.After treatment with E2 at 10~-7 mol/L,the population doubling time of M_3SP_4 cells decreased by 10.8%,clonegenecity increased by 225%, VEGF,c-erbB-2 and Ki-67 expression increased,P16 decreased.In the in vivo assay,the tumour growth increased by 65% and metastasis increased by 609% in nude mice treated with E2 at 0.005 2 mg/d. Conclusion:E2 may stimulate the proliferation and metastasis potential of M_3SP_4 cells.
