Transforming growth factor ?1 induces apoptosis in mouse odontoblast cell line MDPC-23
- VernacularTitle:TGF-?1诱导小鼠成牙本质细胞系MDPC-23细胞凋亡
- Author:
Wenxi HE
;
Zhongying NIU
;
Shouliang ZHAO
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-?1;
MDPC-23;
Apoptosis;
Cell culture
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate if transforming growth factor-?1 (TGF-?1) induces apoptosis in odontoblast cell line MDPC-23. Methods:MDPC-23 cells were treated with TGF-?1 at 0.5,2.5 or 10 ng/ml for 48 h,apoptosis of MDPC-23 cells was detected by annexin V and propidium iodide (PI) staining, cell death detection ELISA and DNA electrophoretic analysis. Results: Consistent results were obtained from three different methods.TGF-?1 at 0.5-10 ng/ml induced apoptosis of MDPC-23 cells in a dose-dependent manner. Conclusion:TGF-?1 may paly a role in apoptosis of odontoblasts.
