The effect of basic fibroblast growth factor and chitosan on human periodontal ligament fibroblasts
- VernacularTitle:bFGF和壳聚糖对人牙周膜成纤维细胞的作用
- Author:
Kaiwen DUAN
;
Mingzhu ZHANG
;
Lu ZHANG
- Publication Type:Journal Article
- Keywords:
Periodontal ligament;
Fibroblasts;
Chitosan;
Fibroblast growth factor 2;
Alkaline phosphatase;
Osteocalcin
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the effect of basic fibroblast growth factor(bFGF) and chitosan(a water soluble derivation) on human periodontal ligament fibroblasts(HPDLFs). Methods:In vitro cultured HPDLFs of passage 5-7 were in culture medium only(group 1), or exposed to 10 ng/ml of bFGF(group 2), 10 ng/ml of bFGF combined with 0.2 mg/ml chitosan(group 3),10 ng/ml of bFGF combined with 2 mg/ml chitosan(group 4),0.2 mg/ml of chitosan(group 5) or 2 mg/ml of chitosan(group 6) for 5 days respectively. Cell proliferation was examined by MTT assay,alkaline phosphatase activity and osteocalcin synthesis were measured by AMP method and radioimmunoassay respectively.Results:Higher proliferation of HPDLFs was observed in group 2 and 3,higher alkaline phosphatase activity in group 5 and 6, and higher osteocalcin synthesis in group 3 and 4.Conclusions:bFGF combined with chitosan(0.2 mg/ml) may increase the proliferation of HPDLFs, stimulate HPDLFs to differentiate into osteoblasts.
