Construction of mcpr1gene vector and expression of mcpr1 in escherichia coli
- VernacularTitle:mcpr1基因原核表达载体的构建及其在大肠杆菌中的表达
- Author:
Dongying XUAN
;
Yan JIN
;
Ming JIN
;
Kun XUAN
;
Rong ZHANG
- Publication Type:Journal Article
- Keywords:
mcpr1 gene;
Fusion protein;
Gene expression;
Purifi cation
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct mcpr1prokaryoti c expression vector and to express MCPR1 protein.Methods:PCR was used to obtain coding region of mcpr1. Construction of a high-level fusion protein expression vector pGEX-4T-mcpr1 was conducted by inserting the fra gment of coding region of mcpr1into a fusion protein expression vector pGEX -4T-1. Then the recombinant plasmid was transferred into E. colito prepar e the MCPR1/GST fusion protein. DNA sequencing and endonucleases digesting were used to check the coding region. Results:pGEX-4T- mcpr1 wa s constructed successfully and the coding region was inserted into the vector co rrectly. A new protein band of 36 000 was observed by SDS-PAGE analysis after i nduction by IPTG. The 36 000 protein amounted to 39 percent of the total prote in and existed mostly in precipitation of broken bacteria. Conclusion: MCPR1 protein can be expressed in E. coliexpression system and purif ied initially.
