The effect of TIP30 on cell cycle distribution of ACC-Mcells
- VernacularTitle:TIP30蛋白在ACC-M细胞中的表达鉴定及作用
- Author:
Lei ZHANG
;
Chuntang LU
;
Zhonghua ZHOU
- Publication Type:Journal Article
- Keywords:
Adenoid cystic carcinorma-M(ACC-M);
TIP30;
Trans fection
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of TIP30 gene on the cell cy cle distribution in adenoid cystic carcinoma ACC-M cells. Methods: Wild type TIP30 gene was transfected into ACC-M cells by using lipofectamin e, the postive cell clone was selected with G418,the expression of TIP30 protei n in the cells was detected by Western blot. Cell cycle distribution was detecte d by flow cytometry. Results:14 days after seletion anti-G418 c ell clone was obtained. Wstern blot revealed that the TIP30 protein was expresse d in the transfected cells.Flow cytometric analysis indicated that TIP30 induced a arrest of the cells in G 0-G 1 phase and led to a decrease of cell percent age in G 2-M and S phase. Conclusion:ACC-M can stably express exogene TIP30.TIP30 can inhibit proliferation of ACC-M cells.
