The effect of different collagen concentration on the proliferation of rat fibroblasts in three-dimensional culture
- VernacularTitle:三维立体培养中不同浓度的胶原凝胶对成纤维细胞生长的影响
- Author:
Hongbing LU
;
Yan JIN
;
Yuan LIU
;
Xiaoliang LIU
;
Rong WANG
;
Yongjie ZHANG
;
Yu ZHAO
- Publication Type:Journal Article
- Keywords:
Collagen;
Fibroblasts;
Three dimensions culture
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of different collagen conc en tration on the proliferation of rat fibroblasts.Methods: Fibrobl asts of 1-2 days new born SD rat from back skin were isolated and seeded into D MEM containing ?=10% fetal boven serum and collagen at the concentration (mg/ml ) of 6.4,5.3and 4.0 respectively. The status and numbers of fibroblasts were observed under light microscope and analysized using ttest. Results : The general condition of fibroblasts in 6.4 mg/ml collagen was better than that in the other groups. The processes of the cells were apparent, simila r to the status of the cells in two-dimensional culture. Vacuoles and granules in cells were observed in 5.3 mg/ml collagen. In 4 mg/ml collagen, the processe s of fibroblasts disappeared; the nuclei were karyopyknosis or dissolved. Some c ells was likely to be apoptotic. The average cell number per 200? field of micr oscope in 6.4,5.3 and 4.0 mg/ml collagen was 25,15 and 6 respectively (P
