Transient expression of human dentin sialoprotein gene in COS-7 cells
- VernacularTitle:人牙本质涎蛋白在COS-7细胞中的表达
- Author:
Ying ZHANG
;
Junnan SHI
;
Ping WANG
- Publication Type:Journal Article
- Keywords:
Sialoglycoproteins;
COS-7 cell;
Transient expression
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To express human dentin sialoprotein (hDSP) gene in COS-7 cells. Methods:hDSP gene was subcloned into mammalian expression vector pcDNA3. The recombined plasmids were transfected into COS-7 cells using lipofectamune PLUS TM kit for transient expression. Western blot analysis and immunohistochemical staining were used to examine the gene products. Results:The constructed vectors were confirmed by digestion with restriction enzyme. An immuno-reaction positive band with relative molecular mass of 60 000 was found by Western blot analysis in culture supernatant and cytoplasms of COS-7 cells. Immunohistochemical staining showed strong positive particles in the cytoplasms. Conclution:hDSP gene can be expressed in COS-7 cells.
