Effects of static tensile strain on the production of prostaglandin E_2 by hum an periodontal ligament fibroblast
- VernacularTitle:静态机械拉伸应变对人牙周膜成纤维细胞前列腺素E_2分泌量的影响
- Author:
Jun HU
;
Xiangming ZHENG
;
Xinming CHEN
- Publication Type:Journal Article
- Keywords:
Periodontal ligament;
Fibroblast;
Prostaglandin E 2;
Tensile strain
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of static tensile strai n on the secretion of prostaglandin E 2 (PGE 2) of human periodontal ligament fibroblasts (PLFs). Methods: Human PLFs were treated by tensile strain values of 0%,8%,12%,16% and 20% for 24, 48 and 72 h respecti vely with a self-devised loading apparatus in vitro. All samples were rando mly allocated into 15 groups and there were 3 in each group. After treatment cu lture media of the samples were collected and the content of PGE 2 in each medi um sample was determined using RIA ELISA. The data analysis was carried out with SPSS using Dunnett test. Results: In group of 0% the sec retion of PGE 2 by PLFs per day had no relation with loading time; E 2 secre tion increased with the increace of loading time and with the tensile strain va lue in the group of 8%,12% and 16%(P
