Detection of Streptococcus sobrinus by polymerase chain reaction
- VernacularTitle:聚合酶链反应(PCR)方法检测远缘链球菌
- Author:
Xianghui XING
;
Fusheng YANG
- Publication Type:Journal Article
- Keywords:
Streptococcus sobrinus;
Streptococcus mutans;
Pol ymerase chain reaction (PCR).
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a PCR method for detecting S treptococcus sobrinus (S.s) in conventional culture. Method: A pair of specific primers were designed from the dexA gene of S.s , the genome DNA of 12 strains of Mutans Streptococci (8 serotypes from a ～h) and 23 species of the bacteria which were commonly found in oral cavity wer e tested by PCR amplification, the PCR products were identified by electrophore sis. Result: Only S.s of serotype d and g could produ ce 277 bp DNA fragments, and the PCR method could detect less than 1 000 copies of S.s. Conclusion: PCR method is specific and sen sitive in the detection of S.s.
