Biological characteristics of cultured rat submandibular gland cells in vitro
- VernacularTitle:体外培养大鼠颌下腺细胞的生物学特性研究
- Author:
Qing ZHOU
;
Songjun FU
;
Yuxin WANG
- Publication Type:Journal Article
- Keywords:
Submandibular gland;
Cell cultivation;
Tissue engineering
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the biological characteristics of cultured salivary gland cells in vitro. Methods: Submandibular gland cells of rat were primarily cultured and subcultured in DMEM supplemented with ?=10% fetal bovine serum. The growth of the cells was studied by cell counting and MTT assay. The morphological characteristic was observed with microscope and transmission electron microscope.The specific protein expression was investigated by immunohistochemical technic. Results:Morpholgy of the cells was epidermoid with well developed organella and zymogen granules.The cells could subultured to 4～5 passages and kept in culture for 30～40 days.The cells of primary culture and passage 2 grew faster than those of passage 4.The cells were CK8.13, keratin and ?-SMA positive. Conclusion:Rat submandibular gland cells can be primarily cultured and subcultured in vitro.The primarily cultured and first subcultured cells may be more potential in growth.
