The effect of leukemia inhibitory factor on the proliferation and differentiation of ectomesenchymal cells of mandibular process in Balb/c fetal mice
- VernacularTitle:白血病抑制因子对Balb/c胎鼠下颌突外胚间充质细胞增殖和分化的影响
- Author:
Manjing DENG
;
Yan JIN
;
Junnan SHI
;
Hongbing LV
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
Ectomesenchymal cell;
Leukemia inhibitory factor;
Proliferation;
Differentiation
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of leukemia inhibitory factor (LIF) on the of proliferation and differentiation of ectomesenchymal cells of mandibular process in Balb/c fetal mice . Methods: Ectomesenchymal cells from the E12.5 mice mandibular process were cultured in DMEM/F12 with 10 6u/L LIF (experimental group) or without LIF (control). The proliferation effect was detected by MTT assay, Brdu test and flow cytometry. Immunohistochemistry were used to identify the differentiation state. Results: By day 7 the A value of the experimental group was 0.38?0.03,that of the control 0.30?0.02 (P
