An in vitro study of three dimensional culture of dog periodontal ligament cells
- VernacularTitle:狗牙周膜细胞三维立体培养的体外实验研究
- Author:
Hong LU
;
Zhifen WU
;
Yu TIAN
;
Shujun CHEN
- Publication Type:Journal Article
- Keywords:
Periodontal ligament cells;
Three-dimensional culture;
Tissue engineering;
Scaffold
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the feasibility of two materials used as scaffolds in periodontal tissue engineering. Methods: Dog periodontal ligament cells cultured in vitro were collected and seeded on three-dimensional framework of cancellous bone matrix (CBM) or nano-HAp/collagen (nHAC) . The cell growth in the scaffolds was observed by cell counting and scanning electronic microscope. Results: Porous structure of the two materials and adhesion and eugonic growth of cells in the scaffolds were observed by scanning electronic microscope.The cell number was doubled in both scaffolds in 72 h culture. Conclusion: It may be feasible to use CBM or nHAC as the scaffolds for periodontal tissue engineering.
