Establishment of an artificial biofilm mouth model for antimicrobial sensitivity tests
- VernacularTitle:人工口腔牙菌斑生物膜药敏实验模型的建立
- Author:
Mingyu LI
;
Zheng LIU
;
Cailian ZHU
- Publication Type:Journal Article
- Keywords:
Microbial sensitivity tests;
Biofilm;
Stneptococcus mutans
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a new antimicrobial sensitivity test model for oral products in vitro. Methods: An artificial biofilm mouth model for antimicrobial sensitivity test was established by modified LKB chromatography chamber. Using sodium fluoride and tea polyphenol as antimicrobial agents and Streptococcus mutans as target, various methodologies on the sensitivity tests were compared. Results: Agar diffusion, agar dilution and broth macro-dilution were more sensitive than modeling biofilms method. The modeling biofilm assay resulted in MIC values of fluoride or tea polyphenol against S. mutans 32 and 12 times higher respectively than the MIC values generated by broth macro-dilution method. Conclusion :The biofilm artificial mouth model may simulate the environment for oral products test.
