Dectection of p16~(CDKN2A) exon 1 methylation in experimental tongue carcinogenesis in rats
- VernacularTitle:实验性大鼠舌癌p16~(CDKN2A)外显子1甲基化状态研究
- Author:
Bin CHENG
;
Yun HONG
;
Chunyang LI
;
Hongbin XIA
;
Linglan YANG
- Publication Type:Journal Article
- Keywords:
p16 CDKN2A;
4-nitroquinoline-1-oxide;
Rat;
Tongue;
Squamous cell carcinoma
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect methylation status of p16 CDKN2A exon 1 during experimental carcinogenesis in rats. Methods:Thirty male clean SD rats were fed with 0.02 g/L of 4-nitroquinoline-oxide (4NQO) in drinking water.13, 16 and 24 weeks after experiment the normal, moderate-severe dysplasia and invasive squamous cell carcinoma tissues were removed from their tongues respectively; then the methylation status of p16 CDKN2A exon 1 were detected by methylation-specific PCR(MSP). Results:A 123 bp-unmethylated product was amplified in all samples but the methylated product was not detected in any of the samples. Conclusion: The p16 CDKN2A exon 1 appeares to be unmethylated during carcinogenesis of tongue cancer in experimental rats.
