Construction of mouse MyoD vector and its expression in Escherichia coli
- VernacularTitle:鼠MyoD原核表达载体的构建及其在大肠杆菌的表达
- Author:
Ruifeng QIN
;
Xiaoming GU
;
Xuerong XING
;
Tianqiu MAO
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Gene expression;
Mice
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the cDNA gene of mouse MyoD by restriction enzyme analysis, and to express the gene in Escherichia coli (E coli) using a protein expression vector.Methods: After the cDNA gene of mouse MyoD had been amplified and identified,it was inserted into expression vector pBV220 in which exogenous gene was controlled by R RP L promoters.The recombinant plasmid pBV-my was transformed into E coli DH5? and the bacteria were induced at 42 ℃ to express encoded protein.Results:The cDNA of mouse MyoD was sequenced correctly.When the engineered bacteria had been induced an anticipated 55 ku protein band from the bacteria was observed on SDS-PAGE gel and amounted to 30% of total bacterial protein. Conclusion:The cDNA of mouse MyoD has been successfully coloned and efficiently expressed in E coli.
