The effects of enamel matrix proteins on the synthesis of alkaline phosphatase and type I collagen in osteoblasts
- VernacularTitle:釉基质蛋白对成骨细胞碱性磷酸酶和I型胶原合成的影响
- Author:
Rong SHU
;
Chunlan WU
- Publication Type:Journal Article
- Keywords:
Enamel matrix proteins;
Osteoblasts;
Alkaline phosphatase;
Collagen type I
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of enamel matrix proteins (EMPs) on the biological characters of osteoblasts.Methods:MC3T3-E1 osteoblasts were cultured in vitro and exposed to EMPs at 300,400 and 500 ?g/ml.Enzyme kinetic method was used to assay alkaline phosphatase (ALP) synthesis and 3H-proline labelling combining collagenase digestion method was used to assay type I collagen synthesis.Results:The results manifested that ALP level in EMPs treated groups was higher than that in control group (P
