Cloning of fadd gene and its apoptosis induction in Tca8113 cells
- VernacularTitle:人fadd基因cDNA的克隆和诱导舌癌细胞凋亡的研究
- Author:
Daqing LIU
;
Zhenqiang SITU
;
Yanming XU
- Publication Type:Journal Article
- Keywords:
Fas associated death domain protein;
Apoptosis;
Tongue;
Carcinoma;
Gene therapy
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the induction of Tca8113 cells to apoptosis by fadd gene.Methods: RT PCR and recombinant PCR were used to amplify human fadd gene and cloned into expression vector pcDNA3 and pIRES2 EGFP, then transfered into Tca8113 cells. The growth and apoptosis of the cells were tested by cell counting,fluorescent microscopy, electron microscopy and flow cytometery. Results: fadd gene was obtained and transfered into Tca8113 cells. After transfection of the gene the growth of the cells was inhibited by 25%～52%, cell number in G 1 phase increased and that in S phase decreased. Apoptosis of the cells was observed. Conclusion: fadd gene can effectively inhibite cell growth and induce Tca8113 cells to apoptosis.
