Histocompoatibility of deantigenic bovine cancellous bone with rabbit osteoblasts

VernacularTitle:牛去抗原松质骨载体的生物相容性研究
Author: Lei PENG ; Yunyu HU ; Zhen WANG
Publication Type:Journal Article
Keywords: Antigen extracted massive bovine concellous bone carrier; Osteoblast; Collagen
From: Journal of Practical Stomatology 2000;0(05):-
CountryChina
Language:Chinese
Abstract: Objective: To prepare surface with collagen, so as to increase the histocompatibility of deantigenic bovine cancellous bone. Methods: Bovine cancellous bone was degreased and macerated by H 2O 2 and decalcified with 0.3 mol/L HCl to form scaffold, surface of which was treaed with collagen, then cultured rabbit osteoblasts were seeded into the scaffold and cultured in vitro . 4, 10, and 21 days later, the cells on the composite materials were observed under sanning election microscope. Results: On day 4, the cells grew in the surface of the scaffold; on day 21, the cells grow into the pores and on all of the surace of the scaffold.Conclusion: Deantigenic bovine cancellous bone with surfaces treated by collagen is histocompatable with rabbit osteoblasts.