Expression and heterogeneity of epidermal growth factor receptor in human salivary gland mucoepidermoid carcinoma MEC-1 cells
- VernacularTitle:人涎腺粘液表皮样癌细胞系的EGFR表达及其异质性的研究
- Author:
Moyi SUN
;
Baolin LIU
;
Yuegui JIANG
- Publication Type:Journal Article
- Keywords:
Salivary glands;
Mucoepidermoid carcinoma;
Epidermal growth factor urogastrone receptor;
Heterogeneity;
Flow cytometer
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expressien and heterogeneity of epidenmoid growth factor receptor(EGFR) in human mucoepidermoid carcinoma MEC 1 cells. Methods: EGFR1 McAb (MaH) immunofluorescent stain and flowcytometer (FCM) were used to detect the positive percentage and heterogeneity of EGFR expression in MEC 1 cells. Results: Positive expression of EGFR was found in 100% of MEC 1 cells. Average peak channel of relative fluorescent intensity was 8.006?4.410. Double peaks of MEC 1 cells from single parameter histogram was observed with FCM. Conclusion: All MEC 1 cells express EGFR and there are heterogeneous cell populations of EGFR expression in MEC 1 cell line.
