Effects of mechanical strain on proliferation of human osteoblast-like cells
- VernacularTitle:机械牵张对人成骨样细胞增殖能力的影响
- Author:
Jing HU
;
Shujuan ZOU
- Publication Type:Journal Article
- Keywords:
Mechanical Strain;
Osteoblasts;
Proliferation
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of mechanical strain on the proliferation of human osteoblasts. Methods: Cloned MG 63 human osteoblasts were cultured and seeded at 10 5/well in 6 well Bioflex cell cuture plates. Subsequently, the cells were exposed to mechanical strain stimulation (at 6%, 12% and 24% elongation rate respectively) for 24 hours using Flexercell 3000 Cell Stretching Unit. MTT colorimetric method was used to assess the proliferation of the cells. Results: The proliferation of MG 63 cells was significantly increased 24 h after mechanical strain treatment( P