The effects of basic fibroblast growth factor, transforming growth factor-?1 and bone morphogenetic protein-2 on the proliferation of rat bone marrow stromal cells
- VernacularTitle:rhbFGF、rhTGF-?、rhBMP-2对骨髓基质细胞体外培养增殖的影响
- Author:
Cailiang LI
;
Hongwei LIU
;
Xiaohua REN
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Basic fibroblast growth factor;
Transforming growth factor ?1;
Bone morphogenetic protein 2;
Cell proliferation
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of human recombinant basic fibroblast growth factor(rhbFGF), recombinant human transforming growth factor ?1 (rhTGF ?1)and recombinant human bone morphogenetic protein 2(rhBMP 2) alone or in combination on the proliferation of rat bone marrow stromal cells(BMSCs) in vitro . Methods: Rat BMSCs were cultured in vitro , rhbFGF(1 ?g/L,f), rhTGF ?1(5 ?g/L, t) and rhBMP 2(200 ?g/L,b) alone or in combination were added into the culture medium, cells were examined by phase contrast microscope, cell growth curves were obtained by cell counting and cell proliferation was detected by MTT assay. Results: 1 ?g/L of rhbFGF alone or combined with 200 ?g/L of rhBMP 2 stimulated BMSC proliferation( P
