The role of parathyroid hormone on the osteocalcin secretion of cultured human dental papilla mesechymal cells
- VernacularTitle:甲状旁腺激素对人牙乳头间充质细胞骨钙素分泌的影响
- Author:
Xinmei CHEN
;
Mingzhen XIAO
;
Longxing NI
;
Jihong FAN
;
Yqing ZHANG
- Publication Type:Journal Article
- Keywords:
Parathyroid hormones;
Dental papilla;
Mesenchymal cell;
Osteocalcin
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of parathyroid hormone(PTH) on the calcification characters of cultured human dental papilla mesenchymal(DPM) cells.Methods: DPM cells were cultured up to 35 days in two groups. The control group was cultured in DMEM supplemented with 15% FCS,50 ?g/ml ascorbic acid and 10 mmol/L ?-glycerophosphate. The cells in experimental group were cultured in above mentionned medium containing 33.3 nmol/L of PTH. The medium was changed every 3 or 4 days. Osteocalcin secretion of the cells was measured by radioimmunoassay.Results: Addition of TPH in medium caused a significant increase of osteocalcin secretion from 21 to 35 days culture (P
