Effects of doxycycline on the proliferation and invasion of adenoid cystic carcinoma SAcc83 cells
- VernacularTitle:强力霉素对腺样囊性癌细胞系SAcc83增殖及侵袭行为的影响
- Author:
Qingshi ZHANG
;
Bin LIU
;
Lianjia YANG
;
Xizhe CHEN
;
Yan CHEN
- Publication Type:Journal Article
- Keywords:
Adenoid cystic carcinoma;
Doxycycline;
Type Ⅳ collagenase;
Invasion
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of doxycycline on the proliferation and invasion of adenoid cystic carcinoma cells.Methods:MTT assay was applied to study the effects of doxycycline on proliferation of SAcc83 cells. Activity of type IV collagenase was detected by the method of gelatin incorporated SDS PAGE electrophoresis.Monolayer cell organ culture was carried out to study the invasion of the cells.Results:Doxycycline at 5～50 ?g/ml inhibited the proliferation of adenoid cystic carcinoma cell in a dose and time dependant manner. 10 ?g/ml of the drug inhibited type Ⅳ collagenase and decreased the invasion of adenoid cystic carcinoma cells by 48%.Conclusion:Doxycycline can inhibit proliferation and invasion of SAcc83 cells.
