The effects of human salivary statherin on adherence of cariogenic bacteria to hydroxyapatite in vitro
- VernacularTitle:唾液富酪蛋白对致龋细菌粘附的影响
- Author:
Jianxia HOU
;
Qiang WANG
;
Ruizhe HUANG
- Publication Type:Journal Article
- Keywords:
Statherin;
Streptococcus mutans;
Streptococcus sanguis;
Hydroxyapatites
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
砄bjective:To investigate the effects of salivary statherin on adherence of two kinds of main cariogenic bacteria. Methods: Human whole salivary statherin was separated and purified by high performance hydrophobic interaction chromatography (HPHIC) and was further identified by SDS PAGE electrophoresis and amino acid analysis.Then the adherence of Streptococcus mutans ( S.mutans serotype c,7H) and Sreptococcus sanguis (S.sanguis ,ATCC 10557)to hydroxyapatite (HA), which was covered with purified statherin or whole saliva (positive control) or PBS buffer (negative control) as experimental pellicles respectively,was studied by bacteria counting.Results:①More S.sanguis adhered to the experimental pellicles than S.mutans ( P
