Effect of recombinant human basic fibroblast growth factor on cell proliferation during mandible fracture healing in rabbit
- VernacularTitle:碱性成纤维细胞生长因子对兔下颌骨骨折愈合中细胞增殖的影响
- Author:
Zhenyu GONG
;
Shuxia ZHOU
;
Jianguang CAO
- Publication Type:Journal Article
- Keywords:
Basic fibroblast growth factor;
Fracture;
Rabbits;
Mandible;
Proliferating cell nuclear antigen;
Immunohistochemistry
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
objective: To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) on cell proliferation during mandible fracture healing. Methods 8 ?g of rhbFGF in 0.3 ml of H 2O was mixed with 1.6 ml of bovine type 1 collagen and then the mixture was vacuumed to be a membrane. The membrane was implanted around the mandibular fracture following the fracture was made in 25 rabbits. 5 animals were sacrificed 1,2,4,8 and 12 weeks respectively after operation. Proliferating cell nuclear antigen (PCNA) in callus was examined with immunohistochemical staining. Type 1 collagen membrane was used as the control in another 25 rabbits. Results:1 week after operation, PCNA positive cell(%) in callus in rhbFGF treated and control group were 65.2?6.1 and 32.0?5.5 respectively ( P
