The effects of mouse recombinant Periostin on the attachment and proliferation of PDL cells on root surfaces
- VernacularTitle:Periostin对人牙周膜细胞在牙根片上附着和生长的影响
- Author:
Ling WAN
;
Zhifen WU
;
Xiaoyang WANG
;
Bin LIU
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Cell adhesion molecule;
Periodontal ligament;
Cells;
Scanning electron microscopy
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of mouse recombinant Periostin on the attachment and proliferation of PDL cells on root surfaces. Methods: Normal (N) and periodontitis (P) root slices were treated with Periostin (Pe) and fibronectin (FN), respectively. Untreated root slices (Co) served as negative controls. PDL cells were seeded on the root slices, the attachment in 24h and the proliferation in 72h of the cells were determined with cell counting. The morphology of the cells was observed under SEM. Results: After 24h cultured, the cells on each mm3 on the root slices of PeN, FNN, CoN, PeP, FNP and CoP were 498. 00?31. 75, 513. 04?27. 52, 432. 88?20. 57, 336. 51 ? 27. 66, 348. 44?30. 23 and 237. 87?32. 54 respectively ; after 72h those were 683. 33? 28. 05, 774. 62?42. 25. 603. 21 ? 19. 76, 510. 52? 28. 31, 579. 19 ? 31. 58 and 445. 92?26. 30 respectively. Conclusion: Periostin can promote the attachment and proliferation of PDL cells on both diseased and healthy root surfaces.
