Effects of hexamethylene bisacetamide on apoptosis and the expression of bcl-2 in human mucoepidermoid carcinoma cell line EEC-1
- VernacularTitle:六亚甲基二乙酰胺对MEC-1细胞凋亡及bcl-2基因表达的影响
- Author:
Lijing WANG
;
Jing WANG
- Publication Type:Journal Article
- Keywords:
Mucoepidermoid carcinoma;
Hexamethylene bisacetamide;
Genes;
bcl-2;
Apoptosis;
Hybridization in situ
- From:
Journal of Practical Stomatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of hexamethylene bisacetamide (HMBA) on apoptosis and the expression of bcl-2 in human mucoepidermoid carcinoma cell line MEC-1. Methods:MEC-1 cells were oxposed to 2 mmol/L HMBA or 1 mg/L 5 -Flourouracil (5 -FU ) for 72 hours. A poptosis index, the expression of bcl-2 protein and bcl-1 mRNA were determined by morphologic observation, terminal dUTP nick and labeling(TUNEL) stain, immunohistochemistry, in situ hybridization and image analysis. Results: Apoptosis indexes(%) in HMBA treated. 5-Fu treated and control cells were 68.5, 23.6 and 2.3 respectevely. The expression of bcl-2 protein and bcl-2 mRNA in HMBA treated cells was higher than in 5-Fu treated or the control cells(p
