Chemical Constituents from Tong An Injection (Ⅰ)
10.11842/wst.2016.12.016
- VernacularTitle:痛安注射液的化学成分研究(Ⅰ)
- Author:
Chan SHANG
;
Haibo LI
;
Mengxuan LI
;
Zhenzhen SU
;
Zhaoqing MENG
;
Wenzhe HUANG
;
Zhenzhong WANG
;
Gang DING
;
Zhonglin YANG
;
Wei XIAO
- Keywords:
Tong-An injection;
flavonoids;
alkaloid;
organic acids;
phenylpropanoids;
prostaglandin E2
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2016;18(12):2118-2124
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at investigating the antiviral constituents from the active fractions of Tong-An (TA) injection.In this study,the active constituents of TA injection were screened by LPS-induced PGE2 production mode to detect the contents of PGE2.The chemical constituents were isolated by HP-20 macroporous resin,silica gel column chromatography,ODS column chromatography,Sephadex LH-20 column chromatography and preparative and semi-preparative HPLC.The structures were identified by spectral data and physicochemical property.As a result,the 95％ ethanol eluate of TA injection on the macroporous adsorption resin column was proved to be the active fraction of TA injection.Seventeen compounds were isolated from TA injection and identified as syringaresinol (1),N-Trans-Feruloyltyramine (2),chelerythrine (3),sinomenine (4),coptisine (5),sanguinarine (6),chelidoniny (7),magnoflorine (8),allocryptopine (9),protopine (10),farrerol (11),dihydrosanguinarine (12),heptadec-(9Z)-enoic acid (13),chlorogenic acid (14),cryptochlorogenin acid (15),3,5-di-O-caffeoylquinic acid (16) and 4,5-di-O-caffeoylquinic acid (17).PGE2 inhibitory activities of these compounds were determined,among which six compounds presented inhibitory activities against PGE2.It was concluded that all the isolated compounds from TA injection were firstly reported with the favorable inhibitory activities of compounds 2,5,9,10,11,12 against PGE2.
