Effects of Celastrus orbiculatus Thunb.Extract on the Overexpression of mTOR in Human HepG2 Cells
10.11842/wst.2016.12.018
- VernacularTitle:南蛇藤提取物对过表达mTOR的人肝癌HepG2细胞株的影响
- Author:
Yayun QIAN
;
Songhua LU
;
Xueyu ZHAO
;
Ting YANG
;
Youyang SHI
;
Feng JIN
;
Yanqing LIU
- Keywords:
Celastrus orbiculatus Thunb.;
hepatic carcinoma;
overexpression;
mammalian target of rapamycin
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2016;18(12):2132-2136
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at investigating the effects of the extract of Chinese herb,Nansheteng (C.orbiculatus Thunb.),on human HepG2 cells through the overexpression of mTOR.The GV238-mTOR recombinant plasmids were transfected into HepG2 cells using molecular biological technology.The expression level of mTOR was evaluated by means of relative activity of luciferase and western blot.Human hepatic carcinoma HepG2/mTOR++ cells were treated with C.orbiculatus extract in different concentrations (20,40,80,160 and 320 tg·mL-1) for 24 h.The mTOR protein expression was detected by western blot.It was found that the protein expression of mTOR in transfected HepG2 cells was significantly enhanced.C.orbiculatus extract significantly inhibited the proliferation of HepG2/mTOR++ cells.Simultaneously,C.orbiculatus extract inhibited mTOR at its protein level in a dose-dependent manner.In conclusion,we successfully constructed recombinant mTOR cloning vectors,and established the stable HepG2 cell line with the overexpression of mTOR.Besides,C.orbiculatus extract significantly inhibited mTOR protein expression in human hepatic carcinoma HepG2 cells.
