Identification of Bovine Hide Gelatine from the Shell Glue of the Red-Eared Slider (Trachemys scripta elegans) Using UPLC-ESI-QTOF-MS
10.11842/wst.2016.12.024
- VernacularTitle:UPLC-ESI-QTOF-MS检测巴西龟龟甲胶中牛皮特征肽的研究
- Author:
Min TANG
;
Jianye YAN
;
Xiaofang ZHAO
;
Bo XU
;
Xuesheng WANG
;
Shunxiang LI
- Keywords:
Ultra-performance liquid chromatography electrospray time of flight-mass spectrometry;
the marker peptides of bovine hide gelatine;
the shell of Trachemys scripta elegans;
tortoise shell glue
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2016;18(12):2165-2169
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at developing an ultra-performance liquid chromatography electrospray time of flight-mass spectrometry (UPLC-ESI-QTOF-MS) method for the identification of bovine hide gelatine from the shell glue of the red-eared slider.Tortoise shell glue was made from the shell of red-eared slider or Reeves' turtle (Chinemys reevesii).The samples were analyzed on UPLC-ESI-QTOF-MS after being digested by trypsin,and the ions at m/z 604.8,m/z 641.3,m/z 790.8 of the marker peptides of bovine hide gelatin were detected.As a result,it was found that the test results of the ions made from the shell of red-eared slider at m/z 641.3 were positive,taking the same fragmentation regularity of MS/MS as the standard of bovine-hide gelatine,while negative results were detected from the ions made from the shell of Reeves' turtle.In conclusion,the method was simple and accurate provided a basis for distinguishing the tortoise shell glue from bovine hide,which could be also used for the quality control of tortoise shell glue.
