Evaluation of genotoxicity of Trois through Ames and in vitro chromosomal aberration tests
10.1016/S2221-1691(13)60176-8
- Author:
Chaudhary MANU
;
Payasi ANURAG
- Publication Type:Journal Article
- Keywords:
Trois;
Ames test;
Chromosomal aberration test
- From:Asian Pacific Journal of Tropical Biomedicine
2013;(11):902-906
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mutagenic potential of Trois using the bacterial reverse mutation assay (Ames test) and in vitro chromosomal aberration test.Methods:typhimurium (TA 98, TA100, TA1535 and TA1537) and Escherichia coli (WP2 uvrA) with and without metabolic activation system (S9 mix) at the dose range of 313 to 5000 μg/plate. Chromosomal aberrations were evaluated in Chinese hamster lung (CHL) cell line at the dose levels of 15, 7.5, 3.7, 1.9 and 0.9 mg/mL in the absence and presence of S9 mix.Results:The ability of Trois to induce reverse mutations was evaluated in Salmonella Trois used in the study with and without S9 mix in all tester strains. Trois did not produce any structural aberration in CHL cells in the presence or absence of S9 mix. There were no increases in the number of revertant colonies at any concentrations of Conclusions: Results of this study suggest that Trois is non-mutagenic.
